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- Title
Development and validation of an RP-HPLC method for quantification of trans-resveratrol in the plant extracts.
- Authors
Cvetkovic, Zika S.; Nikolic, Vesna D.; Savic, Ivan M.; Savic-Gajic, Ivana M.; Nikolic, Ljubisa B.
- Abstract
New, simple, cost effective, accurate and reproducible RP-HPLC method was developed and validated for the quantification of trans-resveratrol in the extracts of grape exocarp and seeds. The method has proved to be simpler and faster than available methods. Methanol was used as a mobile phase with a flow rate of 1.0 cm3 min-1, while the quantification was effected at 306 nm. The separation was performed at 35 °C using a C18 column. The results showed that the peak area response was linear in the concentration range of 1-40 μg cm-3. The values of LOD and LOQ were found to be 0.125 and 0.413 μg cm-3, respectively. The antioxidant activity of the extracts was determined using DPPH assay. The ability of DPPH radicals inhibition decreases in the following order: the extract of grape exocarp > trans-resveratrol standard > the extract of grape seeds.
- Subjects
RESVERATROL; HIGH performance liquid chromatography; PLANT extracts
- Publication
Chemical Industry / Hemijska Industrija, 2015, Vol 69, Issue 6, p679
- ISSN
0367-598X
- Publication type
Article
- DOI
10.2298/HEMIND140917004C