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- Title
Characterization of hydroxyapatite-coated bacterial cellulose scaffold for bone tissue engineering.
- Authors
Ahn, Sung-Jun; Shin, Young; Kim, Se; Jeong, Sung; Jeong, Jin-Oh; Park, Jong-Seok; Gwon, Hui-Jeong; Seo, Da; Nho, Young-Chang; Kang, Seong; Kim, Chong-Yeal; Huh, Jung-Bo; Lim, Youn-Mook
- Abstract
The goal of this study was to develop a novel hydroxyapatite (HA) coated bacterial cellulose (BC) scaffold for bone tissue regeneration. HA-coated BC was prepared by immersing in 30 mL of 5× simulated body fluid at 37°C for 12 h. The resulting HA-coated BC scaffolds were characterized by scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared (ATRFTIR) spectroscopy, and thermal gravimetric analysis (TGA). HA spherical globules were newly formed on the surface of the BC, and a fibrous network of BC scaffolds still maintained their dimensions for cell adhesion and proliferation. ATR-FTIR spectroscopy analysis showed bands assigned to specific signals for phosphate and carbonate ions from HA. HA-coated BC scaffolds of thermal gravimetric analysis presented residue of around 25%. The ability for bone regeneration of HA-coated BC scaffolds was evaluated using a rat calvarial defect model for 4 and 8 weeks. After implantation, both BC and HAcoated BC scaffolds showed new bone formation derived from existing bone, and found new bone even inside the scaffold. Furthermore, a new bone area was signigicantly increased in the HA-coated BC scaffolds compared with those from BC scaffolds, and bone-like materials were frequently found in HA-coated BC scaffolds. Therefore, the HA-coated BC scaffolds can be used as an effective tool for bone tissue regeneration.
- Subjects
HYDROXYAPATITE; TISSUE engineering; THERMOGRAVIMETRY; FOURIER transform infrared spectroscopy; PHOSPHATES; CELL adhesion; CELL proliferation
- Publication
Biotechnology & Bioprocess Engineering, 2015, Vol 20, Issue 5, p948
- ISSN
1226-8372
- Publication type
Article
- DOI
10.1007/s12257-015-0176-z