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- Title
Molecular genetic identification of isolates of the hepatitis A virus (HAV) from monkeys at Adler Primate Center.
- Authors
Dogadov, Dmitriy I.; Korzaya, Lydia I.; Karlsen, Anastasia A.; Kyuregyan, Karen K.
- Abstract
Abstract: Background: The aim of this study was to assess prevalence and genetic variability of hepatitis A virus (HAV) isolates in monkeys born and kept at Adler Primate Center, as well as in imported animals. Methods: The fecal samples from various species of monkeys (n = 119) were studied using reverse transcription seminested PCR, sequencing, and phylogenetic analysis. Results: HAV RNA was detected in 2 <italic>Macaca mulatta</italic> and 1 <italic>Macaca fascicularis</italic> (3.8%) kept at Adler Primate Center (n = 79) and in 11 (27.5%) <italic>Chlorocebus pygerythrus</italic> (n = 40) imported from Tanzania. Phylogenetic analysis demonstrated that all HAV strains belonged to simian genotype V, but differed from the prototype genotype V strain (AGM‐27) by 5.4%‐5.5%. Sequences isolated in this study differed by only 0.1%, suggesting a common source of infection. Conclusions: This study demonstrated the asymptomatic circulation of HAV genotype V among the monkeys at Adler Primate Center, and it indicated the significant genetic diversity within this HAV genotype.
- Subjects
MOLECULAR genetics; HEPATITIS A virus; MONKEY diseases; DISEASE prevalence; PRIMATOLOGY
- Publication
Journal of Medical Primatology, 2018, Vol 47, Issue 2, p87
- ISSN
0047-2565
- Publication type
Article
- DOI
10.1111/jmp.12333