We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Development of a heminested polymerase chain reaction assay for the detection of Haemophilus ducreyi in clinical specimens.
- Authors
Tekle-Michael, Tsegaye; Van Dyck, Eddy; Abdellati, Saïd; Laga, Marie; Tekle-Michael, T; Van Dyck, E; Abdellati, S; Laga, M
- Abstract
Detection of Haemophilus ducreyi in genital ulcer specimens by culture lacks sensitivity. To enhance detection, a heminested polymerase chain reaction (PCR) assay was developed targeting the nucleotide sequence of a gene, designated p27, which encodes for a 27 kDa H. ducreyi-specific protein. The p27 PCR assay detected all (37/37) H. ducreyi strains tested and gave no amplified product from DNA extracts of any of 31 other microorganisms, from 30 non-genital ulcer specimens, or from 29 urethral and vaginal swab specimens collected from non-chancroid STD patients. In genital ulcer disease specimens, compared to combined positive results obtained by culture and a previously described PCR assay, the p27 PCR assay showed a sensitivity of 91% (48/53). The p27 PCR assay provides a specific and a sensitive detection of H. ducreyi in clinical specimens.
- Subjects
POLYMERASE chain reaction; HAEMOPHILUS ducreyi; NUCLEOTIDE sequence; ULCERS; CHANCROID; HAEMOPHILUS diseases; DNA analysis; BACTERIAL protein analysis; COMPARATIVE studies; MALE reproductive organ diseases; HAEMOPHILUS; RESEARCH methodology; MEDICAL cooperation; RESEARCH; EVALUATION research; PREDICTIVE tests
- Publication
International Journal of STD & AIDS, 2001, Vol 12, Issue 12, p797
- ISSN
0956-4624
- Publication type
journal article
- DOI
10.1258/0956462011924425