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- Title
Molecular identification of Trichinella species by multiplex PCR: new insight for Trichinella murrelli.
- Authors
Karadjian, Grégory; Heckmann, Aurélie; La Rosa+, Giuseppe; Pozio, Edoardo; Boireau, Pascal; Vallée, Isabelle
- Abstract
In order to identify Trichinella at the species level, the commonly used test is a multiplex PCR, allowing the discrimination of nine out of the twelve taxa described so far. This test is based on five primer pairs amplifying fragments of the large subunit rDNA. Each taxon produces one or two bands of different sizes, resulting in a specific band pattern. By multiplex PCR, Trichinellamurrelli shows two bands of 127 bp and 316 bp. However, a third band of 256 bp can occur. This band can lead tomisidentification, since it is similar to the 253 bp band displayed by Trichinella britovi. BLAST analysis confirmed that the 256 bp band is from T. murrelli. The aimof this short note is to informanalysts that T.murrelli larvae may display either two- or three-band patterns.
- Publication
Parasite (1252607X), 2017, Vol 24, p1
- ISSN
1252-607X
- Publication type
Article
- DOI
10.1051/parasite/2017053