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- Title
Interleukin-4 ameliorates experimental glomerulonephritis and up-regulates glomerular gene expression of IL-1 decoy receptor.
- Authors
Tam, Frederick W. K.; Smith, Jennifer; Karkar, Aytvian M.; Pusey, Charles D.; Rees, Andrew J.
- Abstract
Interleukin-4 ameliorates experimental glomerulonephritis and upregulates glomerular gene expression of IL-l decoy receptor. Monocytes macrophages and pro-inflammatory cytokines such as interleukin (IL)-1 are important in the pathogenesis of acute glomerulonephritis. The aim of this study was to examine whether IL-4. a cytokine with anti-inflammatory activity, could modulate glomerular inflammation and reduce injury <em>in vivo</em>. Treatment with recombinant rat IL-4 in a model of anti-glomerular basement membrane (GBM) antibody mediated glomerulonephritis in rats reduced glomerular injury. .Albuminuria was less (73% less at day 4) and a lower proportion of glomeruli had capillar) thrombi (79% less at day 4). In IL-4 treated rats, there was a moderate reduction in the number of macrophages in the glomeruli and also suppression of pro-inflammatory activities of the macrophages. Northern blot analysis of glomerular RNA showed that treatment with IL-4 up-regulated mRNA levels of type II IL-1 receptor (IL-1RTII). IL-lRTIl. also known as IL-1 decoy receptor, may act as a decoy molecule to inhibit the effect of IL-lβ . To our knowledge. this is the first demonstration of (i) recombinant IL-4 reducing glomerular inflammation <em>in vivo</em> and (ii) a treatment that increases IL-lRTII expression in association with reduction of tissue injury <em>in vivo</em>.
- Subjects
INTERLEUKIN-4; GLOMERULONEPHRITIS; KILLER cells; GENETIC regulation; MACROPHAGES; MESSENGER RNA; NUCLEIC acids
- Publication
Kidney International, 1997, Vol 52, Issue 5, p1224
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1997.447