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- Title
Protein kinase-X interacts with Pin-1 and Polycystin-1 during mouse kidney development.
- Authors
Xiaohong Li; Hyink, Deborah P.; Radbill, Brian; Sudol, Marius; Haojiang Zhang; Zheleznova, Nadezda N.; Wilson, Patricia D.
- Abstract
The regulation of epithelial branching morphogenesis by bone morphogenetic protein-7 depends, in part, on functionally defined cyclic adenosine monophosphate (cAMP)-dependent protein kinases. We previously identified protein kinase-X (PRKX), a cAMP-dependent kinase, as a regulator of epithelial morphogenesis during kidney development and found that it binds to and phosphorylates Polycystin-1. Overexpression of PRKX stimulates renal epithelial cell migration, tubulogenesis, ureteric bud branching, and glomerular induction in embryonic mouse kidney explants in organ cultures. Here we determined the physiological functions of endogenous PRKX. Knockdown by siRNA of PRKX gene expression in a human fetal collecting tubule (HFCT) cell line exceeded 70% and resulted in decreased cell migration and increased adhesion of the cells to a collagen I matrix. In embryonic mouse kidney explants, the same degree of knockdown decreased ureteric bud branching and glomerular induction. Because PRKX BAG-3 PIN-1 and MAGI-1 are all expressed in ureteric bud derivatives, we tested for interactions among them and found that PRKX binds to all three proteins through its WW domain as determined by TransSignal domain arrays, and it coimmunoprecipitated with Pin-1 in HFCT cell lysates. These studies suggest that Polycystin-1 and Pin-1 may mediate the function of PRKX in kidney development.Kidney International (2009) 76, 54–62; doi:10.1038/ki.2009.95; published online 15 April 2009
- Subjects
KIDNEYS; PROTEIN kinases; BONE morphogenetic proteins; EPITHELIAL cells; MORPHOGENESIS; NEPHROLOGY; MEDICAL research
- Publication
Kidney International, 2009, Vol 76, Issue 1, p54
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.2009.95