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- Title
Downregulation of Melanin Synthesis by Haginin A and Its Application to In Vivo Lightening Model.
- Authors
Jin Hee Kim; Seung Hwa Baek; Dong Hyun Kim; Tae Young Choi; Tae Jin Yoon; Jae Sung Hwang; Mee Ree Kim; Ho Jeong Kwon; Choong Hwan Lee
- Abstract
Haginin A, an isoflav-3-ens isolated from the branch of Lespedeza cyrtobotrya, is almost unknown. Here, we report that haginin A exhibits a strong hypopigmentary effect in Melan-a cells and significantly inhibits melanin synthesis. Haginin A shows potent inhibitory effects with an IC50 (half-maximal inhibitory concentration) value of 5.0 μM on mushroom tyrosinase activity, and functioned as a noncompetitive inhibitor. Also, haginin A decreased microphthalmia-associated transcription factor (MITF), tyrosinase, and tyrosinase-related protein-1 (TRP-1) protein production. To identify the signaling pathway of haginin A, the ability of haginin A to influence extracellular signal-regulated protein kinase (ERK) and Akt/protein kinase B (PKB) activation was investigated. Apparently, haginin A induced ERK and Akt/PKB in a dose-dependent manner. In addition, the specific inhibition of the ERK and the Akt/PKB signaling pathways by PD98059 and LY294002, respectively, increased melanin synthesis. Furthermore, haginin A decreased UV-induced skin pigmentation in brown guinea-pigs. Also, haginin A presented remarkable inhibition on the body pigmentation in the zebrafish model system and decreased tyrosinase activity. Together, haginin A is an effective inhibitor of hyperpigmentation caused by UV irradiation or by pigmented skin disorders through downregulation via ERK and Akt/PKB activation, MITF, and also by the subsequent downregulation of tyrosinase and TRP-1 production.Journal of Investigative Dermatology (2008) 128, 1227–1235; doi:10.1038/sj.jid.5701177; published online 22 November 2007
- Subjects
MELANINS; PHENOL oxidase; PROTEIN kinases; HUMAN skin color; DERMATOLOGY
- Publication
Journal of Investigative Dermatology, 2008, Vol 128, Issue 5, p1227
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1038/sj.jid.5701177