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- Title
Tracing of Helicobacter pylori in the middle ear and mastoid mucosa of patients under 18 years of age with chronic otitis media (with and without cholesteatomas).
- Authors
González-Eslait, Francisco Javier; Blanco-Sarmiento, Pedro Abel; Bejarano-Domínguez, Kabethliz; Barreto, José María; Ruiz-Tejada, Emelina
- Abstract
Objective: It has been estimated that about 5 million people of those affected with otitis media have cholesteatoma, however, its pathophysiology is unclear. In this study we aimed to detect Helicobacter pylori via polymerase chain reaction and real-time polymerase chain reaction in young patients with chronic otitis media. Methods: Patients included in our prospective cross-sectional study had middle-ear/mastoid inflammation and underwent surgical procedures. Middle-ear mucosa samples were collected, and genomic DNA was extracted for H pylori detection by polymerase chain reaction and real-time polymerase chain reaction analyses. Sociodemographic data and gastroesophageal reflux symptoms were analysed. Results: We included 49 patients with mean age of 12.7 ± 3.8 years. Twenty per cent of the patients were diagnosed with cholesteatoma. No increase in H pylori -amplified fluorescence was observed, indicating absence of H pylori. Conclusion: Due to the absence of amplification for H pylori and the fact that albumin was amplified in all samples, we conclude that H pylori does not appear to be a causal factor.
- Subjects
OTITIS media; HELICOBACTER pylori; CROSS-sectional method; GENOMICS; RESEARCH funding; MUCOUS membranes; POLYMERASE chain reaction; CHOLESTEATOMA; MIDDLE ear; AGE distribution; DNA; DESCRIPTIVE statistics; CHRONIC diseases; LONGITUDINAL method; ELECTIVE surgery; MASTOID process; INFLAMMATION; SOCIODEMOGRAPHIC factors; ALBUMINS; GASTROESOPHAGEAL reflux; SYMPTOMS; ADOLESCENCE
- Publication
Journal of Laryngology & Otology, 2024, Vol 138, Issue 5, p503
- ISSN
0022-2151
- Publication type
Article
- DOI
10.1017/S002221512300169X