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- Title
Isolation and cross-amplification of the first set of polymorphic microsatellite markers of two high-Andean cushion plants.
- Authors
Acuña-Rodríguez, Ian S.; Gouin, Nicolas; Cifuentes-Lisboa, Leonardo; Squeo, Francisco A.
- Abstract
In the southern Andes mountains (27-39∘S) Azorella madreporica and Laretia acaulis, two Apiaceae cushion plant species commonly known as yaretas, conform a well-established altitudinal vegetation belt along the lower Andean zone. These species have been considered as fundamental components of several ecological dynamics within their communities; however, high-mountain ecosystems are increasingly threatened worldwide by natural and anthropogenic pressures and the southern Andes are not the exception. Recognizing that genetic information is crucial for the success of any conservation or restoration initiative in wild populations, we developed and cross-amplified 28 specifically designed microsatellite markers (14 in A. madreporica and 14 in L. acaulis), and also tested the cross amplification of 25 markers from the related species Azorella selago. In a region which is particularly vulnerable to global change trends, this new polymorphic microsatellite loci will be useful in the study of the genetic diversity of these high-mountain cushion plants, which are pivotal in the structuring of their native ecosystems.
- Subjects
GENE amplification; MICROSATELLITE repeats in plants; UMBELLIFERAE; ECOSYSTEMS; RESTORATION ecology
- Publication
Journal of Genetics, 2018, Vol 97, Issue 1, pe95
- ISSN
0022-1333
- Publication type
Article
- DOI
10.1007/s12041-018-0999-4