We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Isolation of an Active Compound with Alpha-glucosidase Inhibitory Activity from Amomum xanthioides Wallich.
- Authors
Young-Mee Kim; Myung-Jin Kim; Zhang, Jian Albert; Hee-Jeong Joo; Ji-Yeon Hwang; Jung-In Kim; Young-Sun Choi
- Abstract
α-Glucosidase is the enzyme to digest carbohydrate, and inhibition of α-glucosidase could suppress postprandial hyperglycemia. In the preliminary study methanol extract of Amomum xanthioides Wallich showed strong inhibitory activity against yeast α-glucosidase in vitro. The purpose of this study is to isolate the compound to elicit α-glucosidase inhibitory activity from Amomum xanthioides Wallich which could exert hypoglycemic effect. Methanol extract of Amomum xanthioides Wallich was fractionated with hexane, ethyl acetate, butanol and distilled water successively. Each extract was tested for inhibitory activity against α-glucosidase using para-nitropenyl-α-D-glucopyranoside as a substrate. The ethyl acetate fraction with the strongest inhibitory activity was further purified by Sephadex LH-20 column with 50%, 75% and 100% methanol in sequence as mobile phase and semi-preparative HPLC using C18 column. The peak with the strongest α-glucosidase inhibitory activity was collected for structure analysis by ¹H-NMR and 13C-NMR. We concluded that the active constituent responsible for α-glucosidase inhibitory activity is quercitrin. The inhibitory activity of the isolated quercetrin against yeast α-glucosidase was 55.7% at the concentration of 0.5 mg/mL. The inhibitory activity of acarbose, a commercial α-glucosidase inhibitor was 32.4% at the same concentration. The results suggest that Amomum xanthioides Wallich could be useful as hypoglycemic agent.
- Subjects
HYPOGLYCEMIC agents; GLUCOSIDASE inhibitors; GLUCOSIDASES; CARBOHYDRATES; HYPERGLYCEMIA; BUTANOL; SEPHADEX; ACARBOSE
- Publication
FASEB Journal, 2007, Vol 21, Issue 6, pA1085
- ISSN
0892-6638
- Publication type
Article