We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Occurrence of A-kinase anchor protein and associated cAMP-dependent protein kinase in the inner compartment of mammalian mitochondria
- Authors
Sardanelli, Anna Maria; Signorile, Anna; Nuzzi, Rosanna; Rasmo, Domenico De; Technikova-Dobrova, Zuzana; Drahota, Zdenek; Occhiello, Antonella; Pica, Alessandra; Papa, Sergio
- Abstract
Abstract: Evidence showing the existence in the inner compartment of rat-heart mitochondria of AKAP121 and associated PKA is presented. Immunoblotting analysis and trypsin digestion pattern show that 90% or more of mitochondrial C-PKA, R-PKA and AKAP121 is localized in the inner mitochondrial compartment, when prepared both from isolated mitochondria or cardiomyocyte cultures. This localization is verified by measurement of the specific catalytic activity of PKA, radiolabelling of R-PKA by 32P-phosphorylated C-PKA and of AKAP by 32P-phosphorylated R-PKA and electron microscopy of mitochondria exposed to gold-conjugated AKAP121 antibody.
- Subjects
PROTOPLASM; MITOCHONDRIA; ORGANELLES; EXTRACHROMOSOMAL DNA
- Publication
FEBS Letters, 2006, Vol 580, Issue 24, p5690
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2006.09.020