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- Title
Simultaneous separation of water- and fat-soluble vitamins in isocratic pressure-assisted capillary electrochromatography using a methacrylate-based monolithic column.
- Authors
Yamada, Hiroki; Kitagawa, Shinya; Ohtani, Hajime
- Abstract
A method of simultaneous separation of water- and fat-soluble vitamins using pressure-assisted CEC with a methacrylate-based capillary monolithic column was developed. In the proposed method, water-soluble vitamins were mainly separated electrophoretically, while fat soluble-ones were separated chromatographically by the interaction with a methacrylate-based monolith. A mixture of six water-soluble and four fat-soluble vitamins was separated simultaneously within 20 min with an isocratic elution using 1 M formic acid (pH 1.9)/acetonitrile (30:70, v/v) containing 10 mM ammonium formate as a mobile phase. When the method was applied to a commercial multivitamin tablet and a spiked one, the vitamins were successfully analyzed, and no influence of the matrix contained in the tablet was observed.
- Subjects
VITAMIN synthesis; WATER-soluble vitamins; FAT-soluble vitamins; CAPILLARY electrochromatography; MONOLITHIC reactors; METHACRYLATES; ELECTROPHORESIS
- Publication
Journal of Separation Science, 2013, Vol 36, Issue 12, p1980
- ISSN
1615-9306
- Publication type
Article
- DOI
10.1002/jssc.201201191