We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cell membrane stretch activates intermediate-conductance Ca-activated K channels in arterial smooth muscle cells.
- Authors
Hayabuchi, Yasunobu; Nakaya, Yutaka; Mawatari, Kazuaki; Inoue, Miki; Sakata, Miho; Kagami, Shoji
- Abstract
The aim of this study is to determine the signal transduction of membrane stretch on intermediate-conductance Ca-activated K (IKca) channels in rat aorta smooth muscle cells using the patch-clamp technique. To stretch the cell membrane, both suction to the rear end of patch pipette and hypotonic shock were used. In cell-attached and inside-out patch configurations, the open probability of IKca channels increased when 20- to 45-mmHg suction was applied. Hyposmotic swelling efficiently increased IKca channel current. When the Ca-free solution was superfused, the activation of IKca current by the hyposmotic swelling was reduced. Furthermore, gadolinium (Gd) attenuated the activation of IKca channels induced by hyposmotic swelling, whereas nicardipine did not. In the experiments with Ca-free bath solution, pretreatment with GF109203X, a protein kinase C (PKC) inhibitor, completely abolished the stretch-induced activation of IKca currents. The stretch-induced activation of IKca channels was strongly inhibited by cytochalasin D, indicating a role for the F-actin in modulation of IKca channels by changes in cell stretching. These data suggest that cell membrane stretch activates IKca channels. In addition, the activation is associated with extracellular Ca influx through stretch-activated nonselective cation channels, and is also modulated by the F-actin cytoskeleton and the activation of PKC.
- Subjects
CELL membrane abnormalities; CALCIUM-dependent potassium channels; SMOOTH muscle; CELLULAR signal transduction; ION channels; CYTOSKELETON; PROTEIN kinase C
- Publication
Heart & Vessels, 2011, Vol 26, Issue 1, p91
- ISSN
0910-8327
- Publication type
Article
- DOI
10.1007/s00380-010-0025-0