We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Investigation of rust mycoparasite Sphaerellopsis filum (TNAU SF1) volatilome during mono and di-partite interaction with Puccinia arachidis, causing Peanut rust.
- Authors
Durgadevi, D.; Rajendran, L.; Karthikeyan, G.; Raguchander, T.
- Abstract
The foliar hyperparasite Sphaerellopsis filum TNAU SF1 has biocontrol activity against peanut rust Puccinia arachidis. Of 10 epiphytic mycoparasitic isolates from groundnut rust–infected leaves S. filum TNAU SF1 showed 78% inhibition against P. arachidis. The application of S. filum @ 5 × 106 spores / ml reduced pustules up to 42% and the interaction of the mycoparasite was documented using scanning electron microscopy. The S. filum was entwining around the pathogen mycelium and derived nutrients from uredospores leading to shrivelling of the uredospores. Forty-two microbial volatile organic compounds were found in axenic cultures during interaction of S. filum with P. arachidis. Interestingly, the novel antimicrobial compounds viz., butanoic acid butyl ester, amyl nitrile and pentanol were unique to S. filum. Upon interaction with P. arachidis beta ocimene, Hydroxychalcone, cymene, D-Alanine, and diethyl trisulphide were up regulated. Compounds that produced only minimally against P. arachidis were Nonanal, hexenyl acetate, suggesting virulence component of the pathogen. This study also unraveled the induction of salicylic acid and phenyl propenoid pathways when S. filum interacts with P. arachidis, which is responsible for colonization of the pathogen. The liquid formulation of S. filum TNAUSF1 showed the smallest severity of rust compared to the control. Hence, it could be used as a potential mycoparasite against rust.
- Subjects
STRIPE rust; PUCCINIA; VOLATILE organic compounds; AXENIC cultures; SALICYLIC acid; SCANNING electron microscopy; PEANUTS
- Publication
European Journal of Plant Pathology, 2024, Vol 168, Issue 1, p167
- ISSN
0929-1873
- Publication type
Article
- DOI
10.1007/s10658-023-02742-8