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- Title
Influence of the Escherichia coli oxyR gene function on λ prophage maintenance.
- Authors
Glinkowska, Monika; Łoś, Joanna M.; Szambowska, Anna; Czyż, Agata; Całkiewicz, Joanna; Herman-Antosiewicz, Anna; Wróbel, Borys; Węgrzyn, Grzegorz; Węgrzyn, Alicja; Łoś, Marcin
- Abstract
In Escherichia coli hosts, hydrogen peroxide is one of the factors that may cause induction of λ prophage. Here, we demonstrate that H2O2-mediated λ prophage induction is significantly enhanced in the oxyR mutant host. The mRNA levels for cI gene expression were increased in a λ lysogen in the presence of H2O2. On the other hand, stimulation of the pM promoter by cI857 overproduced from a multicopy plasmid was decreased in the Δ oxyR mutant in the presence of H2O2 but not under normal growth conditions. The purified OxyR protein did bind specifically to the pM promoter region. This binding impaired efficiency of interaction of the cI protein with the OR3 site, while stimulating such a binding to OR2 and OR1 sites, in the regulatory region of the pM promoter. We propose that changes in cI gene expression, perhaps in combination with moderately induced SOS response, may be responsible for enhanced λ prophage induction by hydrogen peroxide in the oxyR mutant. Therefore, OxyR seems to be a factor stimulating λ prophage maintenance under conditions of oxidative stress. This proposal is discussed in the light of efficiency of induction of lambdoid prophages bearing genes coding for Shiga toxins.
- Subjects
ESCHERICHIA coli; HYDROGEN peroxide; INDUCTION (Logic); MESSENGER RNA; OXIDATIVE stress
- Publication
Archives of Microbiology, 2010, Vol 192, Issue 8, p673
- ISSN
0302-8933
- Publication type
Article
- DOI
10.1007/s00203-010-0596-2