We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Simultaneous in vivo truncation of pectic side chains.
- Authors
Bernhard Borkhardt; Jesper Harholt; William Willats; Peter Ulvskov
- Abstract
Abstract Despite the wide occurrence of pectin in nature only a few source materials have been used to produce commercial pectins. One of the reasons for this is that many plant species contain pectins with high levels of neutral sugar side chains or that are highly substituted with acetyl or other groups. These modifications often prevent gelation, which has been a major functional requirement of commercial pectins until recently. We have previously shown that modification of pectin is possible through heterologous expression of pectin degrading enzymes in planta. To test the effect of simultaneous modification of the two main neutral pectic side chains in pectic rhamnogalacturonan I (RGI), we constitutively expressed two different enzymes in Arabidopsis thaliana that would either modify the galactan or the arabinan side chains, or both side chains simultaneously. Our analysis showed that the simultaneous truncation of arabinan and galactan side chains is achievable and does not severely affect the growth of Arabidopsis thaliana.
- Subjects
PECTINS; SUGAR; GELATION; GENE expression in plants; PLANT enzymes; ARABIDOPSIS thaliana; GALACTANS
- Publication
Transgenic Research, 2009, Vol 18, Issue 6, p961
- ISSN
0962-8819
- Publication type
Article