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- Title
Expression and Secretion of CXCL8 (IL-8), Release of Tryptase and Transcription of Histidine Decarboxylase mRNA by Anti-IgE-Activated Human Umbilical Cord Blood-Derived Cultured Mast Cells.
- Authors
Castellani, Maria L.; Shaik, Yasdani B.; Perrella, Alessandro; Frydas, Stavros; Simeonidou, Isaia; Salini, Vincenzo; Tetè, Stefano; Conti, Chiara M.; Vecchiet, Jacopo; Theoharides, Theoharides C.; Conti, Pio; De Lutiis, Maria A.
- Abstract
Activation of cytokine receptors and alterations in cytokines are thought to play important roles in neuronal dysfunction and in the pathogenesis of the nervous system diseases. CXCL8 (IL-8) is a CXC chemokine with chemotactic and inflammatory properties. Chemokines control mast cell infiltration in several inflammatory diseases, including stress and neurological dysfunctions. Using isolated human umbilical cord blood-derived cultured mast cells (HUCMC) from hematopoietic stem cells CD34+, mast cells were immunologically activated with anti-IgE at concentrations of 1, 5, 10 and 20 μg/ml leading to the dose-dependent production of IL-8 (p < 0.05). The increase in IL-8 mRNA expression was also noted when the cells were treated with anti-IgE at 10 μg/ml for 6 h. Immunologically activated HUCMC provoked the generation of tryptase in a dose- and time-dependent manner. We also found increased histidine decarboxylase (HDC) expression in activated HUCMC after 6 h of incubation, a rate-limiting enzyme responsible for the generation of histamine from histidine. Taken together, these results confirm that anti-IgE-activated mast cells release inflammatory mediators including CXCL8, a CXC chemokine which regulates several biological effects of mast cells, e.g. chemoattraction, and possibly causes cell arrest. Copyright © 2007 S. Karger AG, Basel
- Publication
Neuroimmunomodulation, 2007, Vol 14, Issue 2, p97
- ISSN
1021-7401
- Publication type
Article
- DOI
10.1159/000107425