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- Title
Structural polymorphism of I-E subregion antigens determined by a gene in the H-2K to I-B genetic interval.
- Authors
SILVER, JACK; RUSSELL, WILLIAM A.
- Abstract
THE generation of immune responses in mice is influenced by Ir genes located in the I region of the major histocompatibility complex (MHC)1. In some instances maximum responses require complementation by two genes, one in the I-A or I-B and the other in the I-E or I-C subregion2,3. The effects of these genes are thought to be mediated by Ia alloantigens, which are cell surface molecules whose expression is controlled by the I region4. This is based on the observations that anti-Ia sera inhibit in vitro immune responses5,6, and soluble factors that enhance in vitro immune responses express Ia alloantigenic determinants7,9. Jones et al.10, using two-dimensional gel electrophoresis, observed that the expression of I-E subregion antigens is controlled by two genes, one in the I-A subregion, the other in the I-E subregion, and that the polymorphism of these antigens is influenced by an I-A subregion gene. As an explanation, the authors proposed that only one of the two polypeptide chains present in I-E immunoprecipitates is an I-E subregion product, the second being a product of the I-A subregion. Antisera obtained by cross-immunisation of I-E subregion-disparate strains of mice immunoprecipitates a molecular complex consisting of two chains, designated α and β, with molecular weights of 32,000 and 29,000 respectively11-14. Previous studies suggested that I-E antigens isolated from B10.A(5R) and B10.D2 mice had identical α-chains but different β-chains15. However, as these mice differed at multiple genetic regions, it was not possible to show which I subregion(s) determined the polymorphism of the Eβ chain. Therefore, we investigated the effects of the I-A subregion on the polymorphism of I-E subregion antigens. We have now shown by peptide mapping that the I-E subregion polymorphism which Jones et al. found to be controlled by the I-A subregion probably reflects structural polymorphism of β-chains controlled by an I-A subregion gene.
- Publication
Nature, 1979, Vol 279, Issue 5712, p437
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/279437a0