We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Retrograde transport of cholera toxin from the plasma membrane to the endoplasmic reticulum requires the trans-Golgi network but not the Golgi apparatus in Exo2-treated cells.
- Authors
Yan Feng; Jadhav, Ashutosh P.; Rodighiero, Chiara; Fujinaga, Yukako; Kirchhausen, Tomas; Lencer, Wayne I.
- Abstract
Cholera toxin (CT) follows a glycolipid-dependent entry pathway from the plasma membrane through the trans-Golgi network (TGN) to the endoplasmic reticulum (ER) where it is retro-translocated into the cytosol to induce toxicity. Whether access to the Golgi apparatus is necessary for transport to the ER is not known. Exo2 is a small chemical that rapidly blocks anterograde traffic from the ER to the Golgi and selectively disrupts the Golgi apparatus but not the TGN. Here we use Exo2 to determine the role of the Golgi apparatus in CT trafficking. We find that under the condition of complete Golgi ablation by Exo2, CT reaches the TGN and moves efficiently into the ER without loss in toxicity. We propose that even in the absence of Exo2 the glycolipid pathway that carries the toxin from plasma membrane into the ER bypasses the Golgi apparatus entirely.
- Subjects
CHOLERA; TOXINS; BIOCHEMICAL genetics; GOLGI apparatus; ENDOPLASMIC reticulum; CELL membranes
- Publication
EMBO Reports, 2004, Vol 5, Issue 6, p596
- ISSN
1469-221X
- Publication type
Article
- DOI
10.1038/sj.embor.7400152