We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Stachys Species: Comparative Evaluation of Phenolic Profile and Antimicrobial and Antioxidant Potential.
- Authors
Benedec, Daniela; Oniga, Ilioara; Hanganu, Daniela; Tiperciuc, Brîndușa; Nistor, Adriana; Vlase, Ana-Maria; Vlase, Laurian; Pușcaș, Cristina; Duma, Mihaela; Login, Cristian Cezar; Niculae, Mihaela; Silaghi-Dumitrescu, Radu
- Abstract
This study aimed to investigate the polyphenolic composition and antioxidant and antimicrobial potential of six Romanian Stachys species: S. officinalis, S. germanica, S. byzantina, S. sylvatica, S. palustris, and S. recta. The LC-MS/MS method was used to analyze the polyphenolic profile, while the phenolic contents were spectrophotometrically determined. The antioxidant activity was evaluated using the following methods: DPPH, FRAP, nitrite-induced autooxidation of hemoglobin, inhibition of cytochrome c-catalyzed lipid peroxidation, and electron paramagnetic resonance spectroscopy. The in vitro antimicrobial properties were assessed using agar-well diffusion, broth microdilution, and antibiofilm assays. Fifteen polyphenols were identified using LC-MS and chlorogenic acid was the major component in all the samples (1131.8–6761.4 μg/g). S. germanica, S. palustris, and S. byzantina extracts each displayed an intense antiradical action in relation to high contents of TPC (6.40 mg GAE/mL), flavonoids (3.90 mg RE/mL), and caffeic acid derivatives (0.89 mg CAE/mL). In vitro antimicrobial and antibiofilm properties were exhibited towards Candida albicans, Gram-positive and Gram-negative strains, with the most intense efficacy recorded for S. germanica and S. byzantina when tested against S. aureus. These results highlighted Stachys extracts as rich sources of bioactive compounds with promising antioxidant and antimicrobial efficacies and important perspectives for developing phytopharmaceuticals.
- Subjects
ELECTRON paramagnetic resonance spectroscopy; DISC diffusion tests (Microbiology); STACHYS; CAFFEIC acid; CHLOROGENIC acid; ACID derivatives; BIOACTIVE compounds; HYDROXYCINNAMIC acids
- Publication
Antibiotics (2079-6382), 2023, Vol 12, Issue 11, p1644
- ISSN
2079-6382
- Publication type
Article
- DOI
10.3390/antibiotics12111644