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- Title
Frequency of Foxp3<sup>+</sup>CD4<sup>+</sup>CD25<sup>+</sup> T cells is associated with the phenotypes of allergic asthma.
- Authors
MATSUMOTO, Koichiro; INOUE, Hiromasa; FUKUYAMA, Satoru; KAN-O, Keiko; EGUCHI-TSUDA, Miyuki; MATSUMOTO, Takafumi; MORIWAKI, Atsushi; NAKANO, Takako; NAKANISHI, Yoichi
- Abstract
Background and objective: A forkhead/winged-helix family transcriptional repressor, Foxp3, is highly expressed on CD4+CD25+ T regulatory cells. The role of Foxp3+CD4+CD25+ T regulatory cells in asthma remains to be elucidated. Using mouse models and peripheral blood mononuclear cells (PBMC) from subjects with allergic asthma, we aimed to explore whether Foxp3+CD4+CD25+ T regulatory cells associate with asthma phenotypes. Methods: Foxp3+CD4+CD25+ T cells were detected by FACS and the correlation between the frequency of Foxp3+CD4+CD25+ T cells and asthma phenotypes was assessed. Results: The frequency of Foxp3+CD4+CD25+ T cells among total CD4+CD25+ T cells in the lungs showed an inverse correlation with eosinophilic inflammation in BALB/c, A/J and C57BL/6 strains. In addition, the frequency of Foxp3+CD4+CD25+ T cells was inversely correlated with BHR and allergen-specific IgE levels in the serum of A/J mice. In BALB/c mice, the frequency of Foxp3+CD4+CD25+ T cells correlated with the level of IL-10 in BAL fluid. The inverse correlation between the frequency of Foxp3+CD4+CD25+ T cells and eosinophilic inflammation disappeared when mice were treated with anti-IL-10 receptor mAb during allergen challenge. Interestingly, intracellular cytokine staining of lung cells revealed that IL-10 was predominantly produced by Foxp3-CD4+CD25+ T cells. The frequency of Foxp3+CD4+CD25+ T cells among total CD4+CD25+ T cells in PBMC of asthmatics was significantly lower than that of healthy subjects, although there was no significant correlation between the frequency of Foxp3+CD4+CD25+ T cells and asthma severity. Conclusions: These results suggest a role for lung Foxp3+CD4+CD25+ T cells in the regulation of asthma phenotypes, presumably through an IL-10-mediated mechanism.
- Subjects
T cells; PHENOTYPES; ASTHMA; ASTHMATICS; INFLAMMATION; CD antigens
- Publication
Respirology, 2009, Vol 14, Issue 2, p187
- ISSN
1323-7799
- Publication type
Article
- DOI
10.1111/j.1440-1843.2008.01472.x