We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
β1 integrin, ILK and mTOR regulate collagen synthesis in mechanically loaded tendon cells.
- Authors
Mousavizadeh, Rouhollah; Hojabrpour, Payman; Eltit, Felipe; McDonald, Paul C.; Dedhar, Shoukat; McCormack, Robert G.; Duronio, Vincent; Jafarnejad, Seyed Mehdi; Scott, Alex
- Abstract
Tendons are specialized tissues composed primarily of load-responsive fibroblasts (tenocytes) embedded in a collagen-rich extracellular matrix. Habitual mechanical loading or targeted exercise causes tendon cells to increase the stiffness of the extracellular matrix; this adaptation may occur in part through collagen synthesis or remodeling. Integrins are likely to play an important role in transmitting mechanical stimuli from the extracellular matrix to tendon cells, thereby triggering cell signaling pathways which lead to adaptive regulation of mRNA translation and protein synthesis. In this study, we discovered that mechanical stimulation of integrin β1 leads to the phosphorylation of AKT, an event which required the presence of integrin-linked kinase (ILK). Repetitive stretching of tendon cells activates the AKT and mTOR pathways, which in turn regulates mRNA translation and collagen expression. These results support a model in which integrins are an upstream component of the mechanosensory cellular apparatus, regulating fundamental tendon cell functions relevant to exercise-induced adaptation and mechanotherapy.
- Subjects
INTEGRIN-linked kinase; MTOR protein; COLLAGEN; PROTEIN synthesis; TENDONS; FIBROBLASTS
- Publication
Scientific Reports, 2020, Vol 10, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-020-69267-6