We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Anti-thrombosis Effects and Mechanisms by Xueshuantong Capsule Under Different Flow Conditions.
- Authors
Han, Shuxian; Chen, Ying; Wang, Jinyu; Zhang, Qian; Han, Bing; Ge, Yimeng; Xiang, Yanhua; Liang, Rixin; Zhu, Xiaoxin; You, Yun; Liao, Fulong
- Abstract
Xueshuantong capsule (XST) is a patented traditional Chinese medicine used for the prevention and treatment of thrombosis. The molecular mechanism of anti-thrombotic effect of XST was investigated through the cross-talk among the platelets/leukocytes, endothelial cells (ECs), and flow shear stress. The Bioflux 1000 system was used to generate two levels of shear stress conditions: 0.1 and 0.9 Pa. Bioflux Metamorph microscopic imaging system was used to analyze the adhesion cell numbers. Protein expressions were detected by western blotting and flow cytometry. The flow-cytometry results showed that under 0.1 Pa flow, XST decreased ADP induced platelets CD62p surface expression in a concentration-dependent manner. Under 0.9 Pa flow, XST at a concentration of 0.15 g⋅L-1 reduced the platelets activation by 29.5%, and aspirin (ASA) showed no inhibitory effects. XST showed similar efficiency on monocytes adhesion both under 0.1 and 0.9 Pa flow conditions, and the inhibition rate was 30.2 and 28.3%, respectively. Under 0.9 Pa flow, the anti-adhesive effects of XST might be associated with the suppression of VE-cadherin and Cx43 in HUVECs. Blood flow not only acts as a drug transporter, but also exerts its effects to influence the pharmacodynamics of XST. Effects of XST on inhibiting platelets activation and suppressing platelets/leukocytes adhesion to injured ECs are not only concentration-dependent, but also shear stress-dependent. The mechanic forces combined with traditional Chinese medicine may be used as a precise treatment for cardiovascular diseases.
- Subjects
THROMBOSIS; ENDOTHELIAL cells; ENDOTHELIAL seeding; SHEARING force; CYTOMETRY
- Publication
Frontiers in Pharmacology, 2019, pN.PAG
- ISSN
1663-9812
- Publication type
Article
- DOI
10.3389/fphar.2019.00035