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- Title
Two-photon imaging of neuronal activity in motor cortex of marmosets during upper-limb movement tasks.
- Authors
Yuka Hirayama; Shin-Ichiro Terada; Teppei Ebina; Tanaka, Yasuhiro R.; Yoshito Masamizu; Masanori Matsuzaki; Riichiro Hira; Reiko Hirakawa; Atsushi Nambu; Akiya Watakabe; Tetsuo Yamamori; Erika Sasaki; Daisuke Koketsu; Kazuo Hikosaka; Hiroaki Mizukami
- Abstract
Two-photon imaging in behaving animals has revealed neuronal activities related to behavioral and cognitive function at single-cell resolution. However, marmosets have posed a challenge due to limited success in training on motor tasks. Here we report the development of protocols to train head-fixed common marmosets to perform upper-limb movement tasks and simultaneously perform two-photon imaging. After 2-5 months of training sessions, head-fixed marmosets can control a manipulandum to move a cursor to a target on a screen. We conduct two-photon calcium imaging of layer 2/3 neurons in the motor cortex during this motor task performance, and detect task-relevant activity from multiple neurons at cellular and subcellular resolutions. In a two-target reaching task, some neurons show directionselective activity over the training days. In a short-term force-field adaptation task, some neurons change their activity when the force field is on. Two-photon calcium imaging in behaving marmosets may become a fundamental technique for determining the spatial organization of the cortical dynamics underlying action and cognition.
- Subjects
BRAIN imaging; NEURONS; MOTOR cortex; MARMOSET behavior; MOTOR cortex physiology; MOTOR ability
- Publication
Nature Communications, 2018, Vol 9, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-018-04286-6