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- Title
Transketolase Is Identified as a Target of Herbicidal Substance α-Terthienyl by Proteomics.
- Authors
Zhao, Bin; Huo, Jingqian; Liu, Ning; Zhang, Jinlin; Dong, Jingao
- Abstract
α-terthienyl is a natural phytotoxin isolated originally from Flaveria bidentis (L.) Kuntze. The bioassay presented here shows the strong herbicidal activity of α-terthienyl on Digitaria sanguinalis, Arabidopsis thaliana and Chlamydomonas reinhardtii. The α-terthienyl-induced response of A. thaliana at the protein level was analyzed at different times. Changes in the protein expression profiles were analyzed by two-dimensional gel electrophoresis and liquid chromatography tandem mass spectrometry (LC-MS/MS) mass spectrometry. Sixteen protein spots were identified that showed reproducible changes in the expression of at least 2-fold when compared to the control. Among these 16 spots, three were up-regulated and 13 were down-regulated. The decreased expression of several proteins associated with energy production and carbon metabolism suggested that these processes were affected by α-terthienyl. To search for the candidate proteins in this screen, A. thaliana T-DNA mutants of the candidate proteins were used to test their susceptibility to α-terthienyl. Amongst the others, attkl1, a mutant of transketolase, exhibited a significantly lower sensitivity to α-terthienyl when hit compared with Col-0. Based on the identification of the proteins associated with the response to α-terthienyl by proteomics, a candidate target protein transketolase was identified.
- Subjects
TRANSKETOLASE; HERBICIDES; PROTEOMICS; BIOLOGICAL assay; ARABIDOPSIS thaliana; GEL electrophoresis; LIQUID chromatography-mass spectrometry
- Publication
Toxins, 2018, Vol 10, Issue 1, p41
- ISSN
2072-6651
- Publication type
Article
- DOI
10.3390/toxins10010041