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- Title
Negevirus Piura Suppresses Zika Virus Replication in Mosquito Cells.
- Authors
Carvalho, Valéria L.; Prakoso, Dhani; Schwarz, Erika R.; Logan, Tracey D.; Nunes, Bruno Tardelli Diniz; Beachboard, Sarah E.; Long, Maureen T.
- Abstract
We investigated the interaction between the insect-specific virus, Piura virus (PIUV), and the arbovirus Zika virus (ZIKV) in Aedes albopictus cells. We performed coinfection experiments in C6/36 cells. Piura virus (Cor 33 strain, Colombia) and ZIKV (PRVABC58 strain, Puerto Rico) were co-inoculated into C6/36 cells using two multiplicity of infection (MOI) combinations: 0.1 for both viruses and 1.0 for ZIKV, 0.1 for PIUV. Wells were infected in triplicate with either PIUV and ZIKV coinfection, ZIKV-only, or PIUV-only. Mock infected cells served as control wells. The cell suspension was collected daily 7 days post-infection. Zika virus load was titrated by TCID50 on Vero 76 cells. The ZIKV-only infection and PIUV and ZIKV coinfection experiments were also quantified by RT-qPCR. We also investigated whether ZIKV interfered in the PIUV replication. PIUV suppressed the replication of ZIKV, resulting in a 10,000-fold reduction in ZIKV titers within 3 days post-infection. PIUV viral loads were not reduced in the presence of ZIKV. We conclude that, when concurrently infected, PIUV suppresses ZIKV in C6/36 cells while ZIKV does not interfere in PIUV replication.
- Subjects
COLOMBIA; PUERTO Rico; ZIKA virus; VIRAL replication; MOSQUITOES; ZIKA virus infections; MIXED infections; AEDES aegypti; AEDES albopictus
- Publication
Viruses (1999-4915), 2024, Vol 16, Issue 3, p350
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v16030350