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- Title
Structure Characterization and Anti-tumor Activity of Polysaccharides from Rohdea chinensis.
- Authors
Shihao Zhang; Minghua Ma; Sicheng Liu; Guoce Jin; Wencong Li; Yu Zhao; Yanyu Li; Xueyan Li; Zixuan Zhu; Chengxiong Liu; Zhaoxia Liu; Xiaocong Li; Kun Zou
- Abstract
Three crude polysaccharides of RCP-A, B, and C were derived from the rhizome of Rohdea chinensis by means of hot water extraction, gradient ethanol precipitation and dialysis. Three different polysaccharides of RCP-C1-1, RCP-C1-2, and RCP-C1-3 were isolated using cellulose DEAE-52 and Sephadex G-200 chromatography from RCP-C. The average molecular weights of RCP-C1-1, RCP-C1-2, and RCP-C1-3 were measured as 1.51×104, 1.06×104, and 4.86×10³ by means of MALDI-TOF MS and UHPGC, respectively. All three polysaccharides were found to consist of D-fructose and D-glucose following hydrolysis and comparison with literature data. Based on FT-IR and NMR analysis, the polysaccharides were identified as inulin-type fructans, with their backbone composed of α-D-glucopyranosyl-(1→2)-(β-D-fructofuranosyl)n-(1→2)-β-Dfructofuranoside (nRCP-C1-1=79, nRCP-C1-2=58, nRCP-C1-3=27). The anti-tumor activity of the polysaccharides (RCPA, B, and C) was evaluated in H22 tumor-bearing mice. The results suggested that the polysaccharides (RCP-A, RCP-B, and RCP-C) inhibited the growth of H22 hepatocellular. Further, the treated groups of RCP-A, RCP-B, and RCP-C exhibited improvements in body weight as well as spleen/thymus indexes in H22 tumor-bearing mice.
- Subjects
INULIN; POLYSACCHARIDES; ANTINEOPLASTIC agents; FRUCTANS; HOT water; BODY weight; MOLECULAR weights
- Publication
Records of Natural Products, 2024, Vol 18, Issue 3, p339
- ISSN
1307-6167
- Publication type
Article
- DOI
10.25135/rnp.454.2403.3165