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- Title
Maceration-Mediated Liquid–Liquid Extraction and Reverse-Phase High-Performance Liquid Chromatography-Based Pragmatic Analysis of Silybins.
- Authors
Hasany, Syeda Mariam; Huma, Rahila; Akram, Sumia; Ashraf, Rizwan; Mushtaq, Muhammad
- Abstract
This study presents a pragmatic and easily scalable maceration-mediated liquid–liquid extraction (MMLLE) and reverse-phase high-performance liquid chromatography (RP-HPLC)-based determination of Silybins from plant material (Curcuma longa L.). The processing of calibration standards revealed that the RP-HPLC method was linear over a concentration range of 1–100 μg/mL with regression coefficient (R 2) > 0.9950, limit of detection 0.02 μg/mL and limit of quantification <0.07 μg/mL. The optimum chromatographic conditions resolved Silybin A, Silybin B, Isosilybin A and Isosilybin B within 5 min of analysis time. The reproducible recovery rates of spiked flavonolignans (96.24–115.40%) from quality controls established the effectiveness of MMLLE procedure prior to HPLC determination. The real-time analysis revealed the presence of silybins in C. longa roots. The results further endorse that MMLLE prior to chromatographic determination may provide a more pragmatic analytical solution for the analysis/isolation of silybins.
- Subjects
LIQUID-liquid extraction; HIGH performance liquid chromatography; QUALITY control; TURMERIC; ANALYTICAL solutions; DETECTION limit
- Publication
Journal of Chromatographic Science, 2020, Vol 58, Issue 8, p779
- ISSN
0021-9665
- Publication type
Article
- DOI
10.1093/chromsci/bmaa035