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- Title
Quantitative Analysis Combined with Chromatographic Fingerprint for Comprehensive Evaluation of Xiaoer Chaigui Tuire Granules by HPLC-DAD.
- Authors
Hong-Ming Liu; Lei Nie
- Abstract
Quantitative analysis of eight major components combined with chromatographic fingerprint based on high performance liquid chromatography coupled with diode array detector (HPLC-DAD) was developed for the quality evaluation of Xiaoer Chaigui Tuire granules (XCTG), a traditional Chinese medicine (TCM) preparation. Each compound was analyzed by comparing its retention time and UV spectrum of each chromatographic peak with the corresponding retention time and UV spectrum of each standard compound. Baseline separation was achieved on an Agilent Zorbax SB-C18 column with gradient elution of acetonitrile and 0.1% (v/v) phosphoric acid. The developed method was validated by linearity, precision, repeatability, stability and recovery and was subsequently applied to quality evaluation of 12 batches of XCTG with similarity analysis, principal component analysis and cluster analysis. Quantitative analysis combined with HPLC fingerprint could offer an efficient, reliable and practical approach for quality evaluation of XCTG.
- Subjects
CHROMATOGRAPHIC analysis; ANALYTICAL chemistry; CYTOPLASMIC granules; ORGANELLE formation; LIQUID chromatography
- Publication
Journal of Chromatographic Science, 2015, Vol 53, Issue 5, p749
- ISSN
0021-9665
- Publication type
Article
- DOI
10.1093/chromsci/bmu119