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- Title
Apoptosis-inducing factor is a target gene of C/EBPα and participates in adipocyte differentiation
- Authors
Shen, Shao-Ming; Yu, Yun; Wu, Zhao-Xia; Zheng, Ying; Chen, Guo-Qiang; Wang, Li-Shun
- Abstract
Abstract: Besides the contribution in cell death, apoptosis-inducing factor (AIF) also exerts roles in other cellular activities, which are largely unknown. The CCAAT-enhancer binding protein alpha (C/EBPα) is required for differentiation of adipocytes and granulocytes. Here we report that, during 3T3-L1 adipocyte differentiation, AIF expression is robustly upregulated via transcriptional regulation by C/EBPα. The upregulated effect is also confirmed by knockdown and ectopic expression of C/EBPα in U937 and MCF-7 cells respectively with and without endogenous expression of C/EBPα protein. We also reveal that AIF knockdown attenuates 3T3-L1 adipocyte differentiation, presumably due to the mitochondrial respiratory chain deficiency. These results provide evidence for the role of AIF in adipocyte differentiation.
- Subjects
APOPTOSIS; FAT cells; GRANULOCYTES; CELL differentiation; MITOCHONDRIA; TRANSCRIPTION factors; CELL death; GENES
- Publication
FEBS Letters, 2011, Vol 585, Issue 14, p2307
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2011.05.061