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- Title
Metabolism controls dimerization of the chloroplast F<sub>o</sub>F<sub>1</sub> ATP synthase in Chlamydomonas reinhardtii
- Authors
Schwaßmann, Helena J.; Rexroth, Sascha; Seelert, Holger; Dencher, Norbert A.
- Abstract
Abstract: Dimers and oligomers of F-type ATP synthases have been observed previously in mitochondria of various organisms and for the CFoF1 ATP synthase of chloroplasts of Chlamydomonas reinhardtii. In contrast to mitochondria, however, dimers of chloroplast ATP synthases dissociate at elevated phosphate concentration. This suggests a regulation by cell physiological processes. Stable isotope labeling of living cells and blue-native PAGE have been employed to quantitate changes in the ratio of monomeric to dimeric CFoF1 ATP synthase. Chlamydomonas reinhardtii cells were cultivated photoautotrophically in the presence of 15N and photomixotrophically at natural 14N abundance, respectively. As compared to photoautotrophic growth, an increased assembly of ATP synthase dimers on the expense of preexisting monomers during photomixotrophic growth was observed, demonstrating a metabolic control of the dimerization process.
- Subjects
ADENOSINE triphosphatase; ELECTROPHORESIS; MASS spectrometry; SPECTRUM analysis
- Publication
FEBS Letters, 2007, Vol 581, Issue 7, p1391
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2007.02.057