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- Title
In vitro self-propagation of recombinant PrP<sup>Sc</sup>-like conformation generated in the yeast cytoplasm
- Authors
Yang, Wenbin; Yang, Huaiyi; Tien, Po
- Abstract
Abstract: Self-propagation is characteristic property for a prion conformation. Previous studies revealed that prion protein expressed in the cytoplasm gained a PrPSc-like conformation. However, it remains unclear whether the PrPSc-like conformation has the self-propagating property. We found that PrP partially purified from yeast cytoplasm formed amyloid fiber like structures, and we found that the PrPSc-like conformation is able to convert normal PrPC in the brain homogenate to a proteinase K-resistant conformation. These results suggest that yeast cytoplasm expressed recombinant PrPSc-like conformation has the characteristic self-propagating property of a prion, which may have implications in the pathogenesis of sporadic and inherited prion diseases.
- Subjects
CYTOPLASM; YEAST; ELECTRON microscopy; PROTEOLYTIC enzymes
- Publication
FEBS Letters, 2006, Vol 580, Issue 17, p4231
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2006.06.074