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- Title
Cytokine Profile of T lymphocytes from Peripheral Blood and Bronchoalveolar Lavage Fluid in Patients with Active Pulmonary Tuberculosis.
- Authors
Boras, Z.; Jureti, A.; Gagro, A.; Paveli, Lj
- Abstract
The possible immunological relationship between the pattern of Th1/Th2 cytokine production and tuberculin reactivity was assessed in patients with active Mycobacterium tuberculosis infection. The production of the intracellular cytokines interferon (IFN)- γ and interleukin-4 (IL-4) was measured in CD4+ and CD8+ T cells obtained from peripheral blood and bronchoalveolar lavage fluid (BALF) of 20 tuberculin skin-positive patients and compared with the findings recorded in nine tuberculin skin-negative patients with active pulmonary tuberculosis. Upon stimulation with phorbol 12-myristate acetate/ionomycin for 6 h, tuberculin-negative patients had a significantly higher proportion of IFN- γ-producing CD4+ T lymphocytes in BALF than in peripheral blood, while both CD4+ and CD8+ T-lymphocyte subsets in BALF of tuberculin-positive patients secreted more IFN- γ than their peripheral blood counterparts. Tuberculin-negative patients had a significantly higher proportion of IFN- γ-producing CD4+ T lymphocytes in peripheral blood than tuberculin-positive patients. There was no significant difference in the production of IFN- γ by BALF CD4+ T lymphocytes, or by either peripheral blood or BALF CD8+ T lymphocytes. In two tuberculin-negative patients, peripheral blood CD4+ T lymphocytes produced IL-4. Study results suggested a higher immune activity in the blood of tuberculin-negative patients, with an increased lymphocyte activity in BALF versus peripheral blood in both patient groups.
- Subjects
T cells; CYTOKINES; BRONCHOALVEOLAR lavage; TUBERCULOSIS patients; MYCOBACTERIUM tuberculosis
- Publication
Scandinavian Journal of Immunology, 2007, Vol 65, Issue 3, p257
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.2006.01890.x