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- Title
Low frequency of the p53 gene mutations in neuroblastoma.
- Authors
Hosoi, Gaku; Hara, Junichi; Okamura, Takayuki; Osugi, Yuko; Ishihara, Shigehiko; Fukuzawa, Masahiro; Okada, Akira; Okada, Shintaro; Tawa, Akio; Hosoi, G; Hara, J; Okamura, T; Osugi, Y; Ishihara, S; Fukuzawa, M; Okada, A; Okada, S; Tawa, A
- Abstract
<bold>Background: </bold>The p53 gene frequently is affected by point mutations, rearrangements, or deletions that contribute to the genesis or progression of a wide variety of human adult solid tumors; however, to the authors' knowledge, this gene alteration has not been analyzed in neuroblastoma.<bold>Methods: </bold>Genomic DNA samples from 20 children with neuroblastoma, including 16 patients with advanced disease, were screened for the presence of mutations in exons 5-9 of the p53 gene, where over 90% of mutations have been reported to be located in human cancer. The screening technique employed polymerase chain reaction/single-strand conformation polymorphism analysis followed by direct DNA sequencing.<bold>Results: </bold>Heterozygous mutations were detected in 2 of the 20 cases. A silent mutation (T to G transversion) at codon 172 and a missense mutation (G to T transversion) at codon 259 were found in patients with Stage II and Stage IV disease, respectively. Thus, p53 mutations were found to occur in neuroblastoma, but at a low frequency (2 of 20).<bold>Conclusions: </bold>Our data suggest that in a minority of neuroblastomas, p53 gene mutations may play a contributing role in tumorigenesis, but other genes presumably play a major role in this tumor.
- Publication
Cancer (0008543X), 1994, Vol 73, Issue 12, p3087
- ISSN
0008-543X
- Publication type
journal article
- DOI
10.1002/1097-0142(19940615)73:12<3087::AID-CNCR2820731230>3.0.CO;2-9