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- Title
Safety and Immunogenicity of Escherichia coli O157 O-Specific Polysaccharide Conjugate Vaccine in 2-5-Year-Old Children.
- Authors
Ahmed, Amina; Jianpin Li; Shiloach, Yossi; Robbins, John B.; Szu, Shousun C.
- Abstract
Background. Escherichia coli O157:H7 causes severe enteritis and hemolytic-uremic syndrome, mostly in young children and older adults. Similar to the case with Shigella, serum IgG against the O-specific polysaccharide of E. coli O157:H7 may confer immunity by lysing the inoculum in the intestine. A phase 1 trial in adults showed that a vaccine of E. coli O157:H7 O-specific polysaccharide conjugated to recombinant exotoxin A of Pseudomonas aeruginosa (O157-rEPA) was safe and immunogenic. Methods. A phase 2 trial of the O157-rEPA vaccine was conducted in 49 children 2-5 years old who were divided randomly into groups receiving 1 or 2 doses of vaccine. Adverse reactions were monitored. Serum IgG lipopolysaccharide (LPS) antibodies were determined. Results. No significant adverse reactions were observed. At 1 week after the first dose was administered, most children (8 1%) responded with a >4-fold increase in serum IgG LPS antibodies. At 6 weeks after the first dose was administered, all children responded with a >8-fold increase; a second dose did not elicit a booster response. At 26 weeks after the first dose was administered, the geometric mean titer of serum IgG LPS antibodies was ∼20-fold higher than was the prevaccination titer. These serum samples had high titers of bactericidal activity that were correlated roughly with serum IgG LPS antibody titers (r = .78). Conclusions. The 0157-rEPA vaccine was safe and immunogenic in young children. A phase 3 trial of the administration of this conjugate vaccine concurrently with routine immunization in infants is planned.
- Subjects
IMMUNIZATION of children; POLYSACCHARIDES; HUMAN immunogenetics; ESCHERICHIA coli O157:H7; ENTERITIS; HEMOLYTIC-uremic syndrome; SHIGELLA; THERAPEUTICS
- Publication
Journal of Infectious Diseases, 2006, Vol 193, Issue 4, p515
- ISSN
0022-1899
- Publication type
Article
- DOI
10.1086/499821