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- Title
Intracellular Macrophage Growth Rates and Cytokine Profiles of Mycobacterium tuberculosis Strains with Different Transmission Dynamics.
- Authors
TheusZ, Sue A.; Cave, M. Donald; Eisenach, Kathleen D.
- Abstract
Mycobacterium tuberculosis strains associated with 1S6110 restriction fragment-length polymorphism (RFLP) pattern clusters and strains demonstrating unique 1S6110 RFLP patterns were investigated in interferon-γ-activated THP-1 cells by measurement of binding, intracellular growth rate, and cytokine production. Binding was the same for all strains; however, strains from clusters grew significantly more rapidly than did unique strains. Maximal concentration of tumor necrosis factor (TNF)-α was detected at 2 days after infection, with unique strains eliciting significantly greater amounts than did strains from clusters. Interleukin (IL)-10 levels peaked at 1 day after infection with strains from clusters, whereas they peaked at 5 days after infection with unique strains. Rapid growth demonstrated by strains from clusters was highly correlated with rapid production of IL-b and suppression of TNF-α in THP-1 cells during the early stages of infection. Characterization of this phenotype will further advance the investigation of virulence factors in M. tuberculosis.
- Subjects
MYCOBACTERIUM tuberculosis; ANTIVIRAL agents; TUBERCULIN; TUBERCULOSIS transmission; TUMOR necrosis factors; CYTOKINES
- Publication
Journal of Infectious Diseases, 2005, Vol 191, Issue 3, p453
- ISSN
0022-1899
- Publication type
Article