We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Establishment of a near-triploid human B-cell lymphoma cell line with t(14;18) and a p53 gene point mutation.
- Authors
Yamaguchi, Hiroki; Inokuchi, Koiti; Hanawa, Hideki; Tanosaki, Sakae; Shinozawa, Isao; Matuoka, Hiroki; Dan, Kazuo
- Abstract
We report a rare large B-cell non-Hodgkin's lymphoma having a characteristic near-triploid cell population with add(17)(p22) and t(14;18)(q32;q21) translocation. We also established and characterized a new cell line (TK cell) derived from the present lymphoma. A codon 180 mutation (GAG → GAT) in the p53 gene was detected. t(14;18)(q32;q21) was revealed juxtaposition of the bcl-2 and JH genes. Immunoprecipitation analyses of p53 and bcl-2 revealed that abnormality of the p53 protein and aberrant bcl-2 expression, which may protect cells from apoptosis, may be critical to the development of leukaemogenesis exhibiting near-triploid chromosomes.
- Subjects
HODGKIN'S disease; LYMPHOMAS; CHROMOSOME abnormalities
- Publication
British Journal of Haematology, 1999, Vol 105, Issue 3, p764
- ISSN
0007-1048
- Publication type
Article
- DOI
10.1046/j.1365-2141.1999.01402.x