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- Title
Cleavage of cystatin C is not associated with multiple sclerosis.
- Authors
Del Boccio, Piero; Pieragostino, Damiana; Lugaresi, Alessandra; Di Ioia, Maria; Pavone, Barbara; Travaglini, Daniela; D'Aguanno, Simona; Bernardini, Sergio; Sacchetta, Paolo; Federici, Giorgio; Di Ilio, Carmine; Gambi, Domenico; Urbani, Andrea
- Abstract
Recently, Irani and colleagues proposed a C-terminal cleaved isoform cystatin C (12.5kDa) in cerebrospinal fluid as a marker of multiple sclerosis. In this study, we demonstrate that the 12.5kDa product of cystatin C is formed by degradation of the first eight N-terminal residues. Moreover, such a degradation is not specific in the cerebrospinal fluid of multiple sclerosis, but rather is given by an inappropriate sample storage at −20°C. We conclude that the use of the 12.5kDa product of cystatin C in cerebrospinal fluid might lead to a fallacious diagnosis of multiple sclerosis. Preanalytical validation procedure is mandatory for proteomics investigations. Ann Neurol 2007
- Publication
Annals of Neurology, 2007, Vol 62, Issue 2, p201
- ISSN
0364-5134
- Publication type
Article
- DOI
10.1002/ana.20968