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- Title
miR-21-5p 靶向BNC2 调控食管癌的恶性生物行为的研究.
- Authors
邓雨函; 阳梦; 吴振华; 李卉; 热则耶; 麦麦提祖农; 孙晓宏
- Abstract
Objective: To investigate the mechanism by which the identity transcription factor (BNC2) of myoblasts targeted by miR-21-5p in controling the proliferation, migration, and invasion of esophageal cancer (ESCA) cells. Methods: Real-time quantitative polymerase chain reaction (qRT-PCR) was used to assess the relative expression levels of miR-21-5P and BNC2 in esophageal cancer tissues. And any differences in expression were statistically analyzed. Following transfection of ECA109 and KYSE30 esophageal cancer cell lines with miR-21-5p mimics and miR-21-5P inhibitor, and a negative control (NC, including mimcs NC and inhibitor NC), respectively, the cell function test CCK8, and Transwell was used to investigate the impact of miR-21-5p downregulation or overexpression on cell migration, invasion, and proliferation. A bioinformatics technique was utilized to identify and investigate the target genes of miR-21-5P. Then, the dual luciferase assay, Western blot, and qRT-PCR assay were employed to verify the negative regulatory relationship between miR-21-5p and the target genes. Result: In ESCA tumor tissues, it was possible to determine that BNC2 had reduced relative expression levels. and the relative expression levels of miR-21-5p were greater in tumor tissues by qRT-PCR detection. The outcomes of the CCK8 and Transwell studies demonstrated that overexpression of miR-21-5P increased cell invasion, migration, and proliferation. Cell invasion, migration, and proliferation were all decreased by knocking down miR-21-5P. Put differently, miR-21-5P functions as an oncogene in esophageal cancers. The bioinformatics technique predicted BNC2 to be the target gene of miR-21-5P. According to the results of the dual luciferase assay, miR-21-5P was targeted to wild-type BNC2 '-UTR. Furthermore, miR-21-5p mimics were shown to be able to drastically lower the BNC2 gene level by western blot and qRT-PCR. On the other hand, a miR-21-5p inhibitor may dramatically raise BNC2 expression in esophageal cancer cells. These findings suggest a link of targeting between miR-21-5P and BNC2.Conclusion: In ESCA, miR-21-5p targets BNC2, and its regulatory role may contribute to cancer proliferation, migration, and invasion.
- Publication
Journal of Hainan Medical University, 2024, Vol 30, Issue 16, p1236
- ISSN
1007-1237
- Publication type
Article
- DOI
10.13210/j.cnki.jhmu.20240510.001