We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Impact of phloroglucinol and indoleamines on growth and accumulation of podophyllotoxin in the roots of Hyptis suaveolens (L.) Poit.
- Authors
Coelho, Adriane Duarte; Mendonça, Simony Carvalho; Assis, Rafael Marlon Alves de; Rocha, João Pedro Miranda; Shukla, Mukund Rasiklal; Saxena, Praveen Kumar; Bertolucci, Suzan Kelly Vilela; Pinto, José Eduardo Brasil Pereira
- Abstract
Hyptis suaveolens (L.) Poit is known for containing podophyllotoxin (PTOX), a natural lignan used as the lead compound in antitumor agent preparations, in its roots. The use of phloroglucinol (PHL) and indoleamines, such as melatonin (MEL) and serotonin (SER), in plant tissue culture is a promising alternative for eliciting rare and high-value secondary metabolites of medical importance. Aimed to evaluate the impact of different concentrations of PHL, either alone or in combination with MEL or SER, on the growth and accumulation of PTOX in H. suaveolens. Apical segments were vertically inoculated in MS medium containing different concentrations of MEL or SER (0; 0.25; 0.50; 1.00; 5.00 and 10.00 µM) and PHL (0 and 50 mg L-1). After 40 days, growth parameters, chlorophyll content and PTOX content were assessed. Plantlet rooting and root dry weight notably increased with the use of 0.5 µM MEL alongside PHL. Overall, treatments containing PHL showed superior results, except for chlorophyll content. SER supplementation was effective in increasing root growth, root number and leaf area. The combination of 1.0 µM MEL or SER and 50 mg L-1 PHL increased the production of PTOX in the roots of H. suaveolens by 2.0 and 1.6 times, respectively. In conclusion, the application of PHL and indoleamines demonstrated an eliciting effect on both growth and PTOX production in H. suaveolens roots, presenting a promising avenue for further research and application in eliciting targeted secondary metabolites.Key message: The application of phloroglucinol and indoleamines, such as melatonin (MEL) and serotonin (SER), demonstrated an eliciting effect on both growth and podophylotoxin production in Hyptis suaveolens roots.
- Publication
Plant Cell, Tissue & Organ Culture, 2024, Vol 158, Issue 3, p1
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-024-02845-y