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- Title
Anti-TCP1 Antibody Is a Potential Biomarker for Diagnosing Systemic Lupus Erythematosus.
- Authors
Lee, Sang-Won; Baek, Wook-Young; Park, So-Won; Chung, Jee-Min; Park, Ji-Hyun; Kang, Ho Chul; Jung, Ju-Yang; Suh, Chang-Hee
- Abstract
Systemic lupus erythematosus (SLE) is a chronic inflammatory disease caused by autoantibodies. Serum samples from patients with SLE (n = 10) were compared with those from normal controls (NCs, n = 5) using 21K protein chip analysis to identify a biomarker for SLE, revealing 63 SLE-specific autoantibodies. The anti-chaperonin-containing t-complex polypeptide-1 (TCP1) antibody exhibited higher expression in patients with SLE than in NCs. To validate the specificity of the anti-TCP1 antibody in SLE, dot blot analysis was conducted using sera from patients with SLE (n = 100), rheumatoid arthritis (RA; n = 25), Behçet's disease (BD; n = 28), and systemic sclerosis (SSc; n = 30) and NCs (n = 50). The results confirmed the detection of anti-TCP1 antibodies in 79 of 100 patients with SLE, with substantially elevated expression compared to both NCs and patients with other autoimmune diseases. We performed an enzyme-linked immunosorbent assay to determine the relative amounts of anti-TCP1 antibodies; markedly elevated anti-TCP1 antibody levels were detected in the sera of patients with SLE (50.1 ± 17.3 arbitrary unit (AU), n = 251) compared to those in NCs (33.9 ± 9.3 AU), RA (35 ± 8.7 AU), BD (37.5 ± 11.6 AU), and SSc (43 ± 11.9 AU). These data suggest that the anti-TCP1 antibody is a potential diagnostic biomarker for SLE.
- Subjects
BEHCET'S disease; SYSTEMIC lupus erythematosus; PROTEIN microarrays; ENZYME-linked immunosorbent assay; SYSTEMIC scleroderma; AUTOANTIBODIES
- Publication
International Journal of Molecular Sciences, 2024, Vol 25, Issue 16, p8612
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms25168612