We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
In Vivo Antitumor Effects of MK615 Led by PD-L1 Downregulation.
- Authors
Yanaki, Masashi; Kobayashi, Masayuki; Aruga, Atsushi; Nomura, Minoru; Ozaki, Makoto
- Abstract
Background/Aim: MK615 extracted from Prunus mume was reported to have anti-inflammatory effects. In this article, we examined the in vivo antitumor effect of MK615 (an extract from Japanese apricot) using mouse tumor xenografts and focusing on the downregulation of PD-L1 (programmed death-ligand 1), a ligand of programmed cell death-1, a surface protein of activated T cells. Materials and Methods: B16/BL6 melanoma cells were injected into C57BL/6 or BALB/c-nu/nu mice to establish lung metastasis. BALB/c-nu/nu mice (nude mice) were used as a T cell–deficient model. The mice were given MK615 or saline orally every other day for approximately 8 weeks, and their survival was observed. NF-κB (nuclear factor-κB) and PD-L1 expressions of metastatic lung tissues were also examined. Results: The survival rate was improved only in the MK615-treated C57BL/6 mice (P < .05), not in the saline-given control mice or BALB/c-nu/nu mice. The downregulations of NF-κB and PD-L1 were observed in both MK615-treated C57BL/6 and BALB/c-nu/nu mice. These results suggest that the antitumor effects of MK615 are associated with T cell–mediated immunity activated by MK-615-induced PD-L1 downregulation in tumor cells. Conclusion: MK615 is beneficial for a prolonged host survival time in the B16/BL6 melanoma xenograft model associated with T cell–mediated antitumor immunity.
- Subjects
ANIMAL experimentation; ANTIGENS; APRICOT; BIOCHEMISTRY; CELL lines; GENE expression; LUNGS; LUNG tumors; PHENOMENOLOGY; MELANOMA; METASTASIS; MICE; ORAL drug administration; T cells; XENOGRAFTS; DNA-binding proteins; PLANT extracts; CANCER cell culture; IN vivo studies; PROGNOSIS
- Publication
Integrative Cancer Therapies, 2018, Vol 17, Issue 3, p646
- ISSN
1534-7354
- Publication type
Article
- DOI
10.1177/1534735418766403