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- Title
A gene encoding Achlya bisexualis β-amylase and its expression in Saccharomyces cerevisiae.
- Authors
Hee-Ok Kim; Jeong-Nam Park; Dong-Jun Shin; Hwanghee Blaise Lee; Soon-Bai Chun; Suk Bai
- Abstract
Part of a β-amylase genomic DNA sequence from the oomycete, Achlya bisexualis was cloned by polymerase chain reaction (PCR) using degenerate oligonucleotide primers derived from the conserved regions of other known β-amylase sequences. The 5′- and 3′-regions of the β-amylase gene were amplified by genome walking method. The Ach. bisexualis β-amylase gene consisted of a 1338 bp open reading frame, encoding a protein of 446 amino acids with a molecular weight of 49 381 Da, and was not interrupted by any intron. The deduced amino acid sequence of the β-amylase gene had 67% similarity to the β-amylase of Saprolegnia ferax, followed by 40% similarity to that of Arabidopsis thaliana. The β-amylase gene was expressed in Saccharomyces cerevisiae placing it under the control of the alcohol dehydrogenase gene (ADC1) promoter.
- Subjects
POLYMERASE chain reaction; GENETICS; AMINO acids; ORGANIC acids; ARABIDOPSIS; DEHYDROGENASES
- Publication
Biotechnology Letters, 2001, Vol 23, Issue 14, p1101
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1010503731486