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- Title
Histochemische Untersuchungen von Oxydoreduktasen und Esterasen im Perikaryon nach Axondurchtrennung.
- Authors
Thomas, E.; Exss, R.
- Abstract
By means of histochemistry one can observe changes of enzyme activity in the nerve cells after 'primäre Reizung' ( Nissl). We studied in unfixed cold microtome sections the motor neurons of the lumbar region of mice after ischiadectomy; it was possible to cut the unprepared spinal cord within the vertebral column in a cryostat. Changes in the perikaryon become visible 4 days after axotomy. We observed the following details: 1. A number of dehydrogenases (DPN diaphorase, TPN diaphorase, lactate dehydrogenase, β-hydroxybutyrate dehydrogenase, isocitrate dehydrogenase, glutamate dehydrogenase, malate dehydrogenase, glucose-6-phosphate dehydrogenase) shows a rapid and significant increase in concentration which remains unchanged for about 6 weeks. 2. Cytochrome oxidase, succinate dehydrogenase und α-glycerophosphate dehydrogenase which are only visible in small amounts or cannot be found in the perikaryon do not show an increased reaction. 3. A loss of enzyme activity is found for acetylcholine esterase. - Chromatolysis is only poorly visible in the cold microtome sections. But one sees an enlargement of the rounded cell body, a dislocated nucleus, and an enlarged nucleolus. The reaction of the nerve cell after axotomy is observed as an activation of the cytoplasm in respect to certain metabolic functions. There is no increase of enzymes of the terminal oxidation. The decrease of acetylcholine esterase may be interpreted as a sign of a lost function of the neuron.
- Publication
Acta Neuropathologica, 1967, Vol 7, Issue 3, p253
- ISSN
0001-6322
- Publication type
Article
- DOI
10.1007/BF00686376