We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Monitoring Metallo‐Macromolecular Assembly Equilibria by Ion Mobility‐Mass Spectrometry.
- Authors
Endres, Kevin J.; Xie, Ting‐Zheng; Chakraborty, Sourav; Hoopingarner, Chad; Wesdemiotis, Chrys
- Abstract
Ion mobility–mass spectrometry (IM‐MS) allows the separation of isomeric and isobaric species on the basis of their size, shape, and charge. The fast separation timescale (ms) and high sensitivity of these measurements make IM‐MS an ideally suitable method for monitoring changes in macromolecular structure, such as those occurring in interconverting terpyridine‐based metallosupramolecular self‐assemblies. IM‐MS is used to verify the elemental composition (size) and architecture (shape) of the self‐assembled products. Additionally, this article demonstrates its applicability to the elucidation of concentration‐driven association–dissociation (fusion–fission) equilibria between isobaric structures. IM‐MS enables both quantitative separation and identification of the interconverting complexes as well as derivation of the corresponding equilibrium constants (i.e., thermodynamic information) from extracted IM‐MS abundance data. Electrospray ionization coupled to ion mobility‐mass spectrometry enables the separation and quantitative analysis of isobaric coordinatively bound self‐assemblies and permits monitoring their concentration changes during supramolecular fusion–fission equilibria as well as determining the equilibrium constants of the interconverting reactions.
- Subjects
EQUILIBRIUM; SPECTROMETRY
- Publication
Macromolecular Rapid Communications, 2019, Vol 40, Issue 6, pN.PAG
- ISSN
1022-1336
- Publication type
Article
- DOI
10.1002/marc.201800667