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- Title
Quenching of chlorophyll triplet states by carotenoids in algal light-harvesting complexes related to fucoxanthin-chlorophyll protein.
- Authors
Khoroshyy, Petro; Bína, David; Gardian, Zdenko; Litvín, Radek; Alster, Jan; Pšenčík, Jakub
- Abstract
We have used time-resolved absorption and fluorescence spectroscopy with nanosecond resolution to study triplet energy transfer from chlorophylls to carotenoids in a protective process that prevents the formation of reactive singlet oxygen. The light-harvesting complexes studied were isolated from Chromera velia, belonging to a group Alveolata, and Xanthonema debile and Nannochloropsis oceanica, both from Stramenopiles. All three light-harvesting complexes are related to fucoxanthin-chlorophyll protein, but contain only chlorophyll a and no chlorophyll c. In addition, they differ in the carotenoid content. This composition of the complexes allowed us to study the quenching of chlorophyll a triplet states by different carotenoids in a comparable environment. The triplet states of chlorophylls bound to the light-harvesting complexes were quenched by carotenoids with an efficiency close to 100%. Carotenoid triplet states were observed to rise with a ~5 ns lifetime and were spectrally and kinetically homogeneous. The triplet states were formed predominantly on the red-most chlorophylls and were quenched by carotenoids which were further identified or at least spectrally characterized.
- Subjects
CHLOROPHYLL spectra; CHLOROPHYLL analysis; LIGHT-harvesting complex (Photosynthesis); PHOTOSYSTEMS; CAROTENOIDS; ENERGY harvesting
- Publication
Photosynthesis Research, 2018, Vol 135, Issue 1-3, p213
- ISSN
0166-8595
- Publication type
Article
- DOI
10.1007/s11120-017-0416-5