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- Title
Increased cytosolic free sodium in platelets from patients with early-stage chronic renal failure.
- Authors
Tepel, M.; Bauer, S.; Kegel, M.; Raffelsiefer, A.; Wischniowski, H.; Zidek, W.
- Abstract
Cytosolic free sodium concentration ([Na]i) and sodium transport systems were measured in intact platelets from 19 patients with early-stage chronic renal failure and 33 healthy control subjects using the novel fluorescent dye sodium-binding-benzofuran-isophthalate. Resting [Na]i was significantly greater in patients with chronic renal failure compared to control subjects (40.8±3.1mmol/1 versus 32.2±2.0 mmol/1, mean±SEM, <0.05). After inhibition of Na-K-ATPase by 1 mmol/1 ouabain a higher net sodium influx was observed in platelets from patients with chronic renal failure compared to control subjects (49.8±8.7 mmol/1 versus 28.5±5.2 mmol/1, <0.05). The platelet Na-H exchanger was similar in the two groups. Cytosolic free calcium concentration ([Ca]i) was measured using fura2 and did not show significant differences between the two groups. To evaluate whether a circulating factor may be associated with elevated [Na]i, a linked-enzyme Na-K-ATPase assay was included. Compared to control subjects plasma from patients with chronic renal failure produced a significant inhibition of steady-state Na-K-ATPase activity by 11.2±3.0% (<0.01). It is concluded that early-stage renal failure is associated with significant impairment of platelet sodium metabolism.
- Publication
Nephrology Dialysis Transplantation, 1994, Vol 9, Issue 1, p27
- ISSN
0931-0509
- Publication type
Article