We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Preparation of silicon quantum dot–protein complex and application in antibacterial, antibiofilm, and bacterial cell imaging.
- Authors
Zhao, Dan; Jiao, Yan; Liu, Huan; Li, Xiaoyun; Xu, Mengyu; Xiao, Xincai; Zhao, Haiyan
- Abstract
Bacterial and biofilm infections pose great challenges to clinical treatments. This study reports the application of long-wavelength-emitting silicon quantum dots (SiQDs) as antibacterial and antibiofilm agents, and for multi-colored bacterial imaging. Several SiQDs–protein complexes were prepared and screened to obtain high-efficient antibacterial nanocomplex, and SiQDs–polylysin (SiQDs–PLL) exhibited the best antibacterial behavior with minimum inhibitory concentration values against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) at 10 and 15.62 μg mL−1, respectively, superior to the ability of pure SiQDs. Compared to Cu2+ and Mn2+, Zn2+ combined with SiQDs–PLL also showed enhanced antibacterial activity because of the synergistic effect. Furthermore, compared to SiQDs, SiQDs–PLL showed an excellent inhibitory effect against the S. aureus biofilm, by completely inhibiting its growth and achieving 62.13% biofilm removal rate at the concentrations of 60 and 200 μg mL−1, respectively. The antibacterial mechanisms of SiQDs and SiQDs–PLL were explored: SiQDs and SiQDs–PLL exerted antibacterial effects by destroying the bacterial cell membrane and promoting the leakage of cell content (DNA and RNA), thus exhibiting the potential as ideal candidates as antimicrobial agents in clinical settings. SiQDs and SiQDs–PLL also exhibit potential in bacterial imaging due to their excellent optical properties and low cytotoxicity.
- Subjects
CELL imaging; BACTERIAL cells; BACTERIAL cell membranes; ESCHERICHIA coli; QUANTUM dots; SCHIFF bases
- Publication
Journal of Materials Science, 2024, Vol 59, Issue 16, p6946
- ISSN
0022-2461
- Publication type
Article
- DOI
10.1007/s10853-024-09622-4