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- Title
Histological and SEM analysis of root cementum following irradiation with Er:YAG and CO lasers.
- Authors
Almehdi, Aslam; Aoki, Akira; Ichinose, Shizuko; Taniguchi, Yoichi; Sasaki, Katia; Ejiri, Kenichiro; Sawabe, Masanori; Chui, Chanthoeun; Katagiri, Sayaka; Izumi, Yuichi
- Abstract
Recently, the Er:YAG and CO lasers have been applied in periodontal therapy. However, the characteristics of laser-irradiated root cementum have not been fully analyzed. The aim of this study was to precisely analyze the alterations of root cementum treated with the Er:YAG and the CO lasers, using non-decalcified thin histological sections. Eleven cementum plates were prepared from extracted human teeth. Pulsed Er:YAG laser contact irradiation was performed in a line at 40 mJ/pulse (14.2 J/cm/pulse) and 25 Hz (1.0 W) under water spray. Continuous CO laser irradiation was performed in non-contact mode at 1.0 W, and ultrasonic instrumentation was performed as a control. The treated samples were subjected to stereomicroscopy, scanning electron microscopy (SEM), light microscopy and SEM energy dispersive X-ray spectroscopy (SEM-EDS). The Er:YAG laser-treated cementum showed minimal alteration with a whitish, slightly ablated surface, whereas CO laser treatment resulted in distinct carbonization. SEM analysis revealed characteristic micro-irregularities of the Er:YAG-lased surface and the melted, resolidified appearance surrounded by major and microcracks of the CO-lased surface. Histological analysis revealed minimal thermal alteration and structural degradation of the Er:YAG laser-irradiated cementum with an affected layer of approximately 20-μm thickness, which partially consisted of two distinct affected layers. The CO-lased cementum revealed multiple affected layers showing different structures/staining with approximately 140 μm thickness. Er:YAG laser irradiation used with water cooling resulted in minimal cementum ablation and thermal changes with a characteristic microstructure of the superficial layer. In contrast, CO laser irradiation produced severely affected distinct multiple layers accompanied by melting and carbonization.
- Subjects
CEMENTUM; RARE earth lasers; CARBON monoxide lasers; HISTOLOGY; SCANNING electron microscopy; ROOT canal treatment; IRRADIATION; DENTAL extraction; MICROSTRUCTURE
- Publication
Lasers in Medical Science, 2013, Vol 28, Issue 1, p203
- ISSN
0268-8921
- Publication type
Article
- DOI
10.1007/s10103-012-1110-7